FineTest
SKU:EH1957
Human VCL(Vinculin) ELISA Kit
Human VCL(Vinculin) ELISA Kit
Couldn't load pickup availability
Product Name
Human VCL(Vinculin) ELISA Kit
Alias
VCL ELISA Kit, Vinculin ELISA Kit, MVCL ELISA Kit, CMD1W ELISA Kit, CMH15 ELISA Kit, Metavinculin ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
VCL ELISA Kit allows for the in vitro quantitative determination of VCL concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of VCL and the recovery rates were calculated by comparing the measured value to the expected amount of VCL in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
92-99 |
96 |
|
EDTA plasma(n=5) |
86-100 |
95 |
|
heparin plasma(n=5) |
85-96 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VCL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-104% |
87-103% |
85-90% |
|
EDTA plasma(n=5) |
87-100% |
90-99% |
82-99% |
|
heparin plasma(n=5) |
84-96% |
81-99% |
86-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
