FineTest
SKU:EH1897
Human DEFA5(Defensin Alpha 5, Paneth Cell Specific) ELISA Kit
Human DEFA5(Defensin Alpha 5, Paneth Cell Specific) ELISA Kit
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Product Name
Human DEFA5(Defensin Alpha 5, Paneth Cell Specific) ELISA Kit
Alias
DEFA5 ELISA Kit, Defensin-5 ELISA Kit, Defensin ELISA Kit, alpha 5 ELISA Kit, HD5 ELISA Kit, 20-94 ELISA Kit, DEF5 ELISA Kit, HD-5 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
DEFA5 ELISA Kit allows for the in vitro quantitative determination of DEFA5 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of DEFA5 and the recovery rates were calculated by comparing the measured value to the expected amount of DEFA5 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-105 |
92 |
|
EDTA plasma(n=5) |
88-104 |
96 |
|
heparin plasma(n=5) |
85-99 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DEFA5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-105% |
85-105% |
91-103% |
|
EDTA plasma(n=5) |
85-101% |
87-99% |
83-101% |
|
heparin plasma(n=5) |
82-99% |
80-96% |
84-93% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
