FineTest
SKU:EH1864
Human NEBL(Nebulette) ELISA Kit
Human NEBL(Nebulette) ELISA Kit
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Product Name
Human NEBL(Nebulette) ELISA Kit
Alias
NEBL ELISA Kit, Nebulette ELISA Kit, LNEBL ELISA Kit, Actin-binding Z-disk protein ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
NEBL ELISA Kit allows for the in vitro quantitative determination of NEBL concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of NEBL and the recovery rates were calculated by comparing the measured value to the expected amount of NEBL in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-104 |
99 |
|
EDTA plasma(n=5) |
85-102 |
92 |
|
heparin plasma(n=5) |
87-102 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NEBL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
91-105% |
89-104% |
88-105% |
|
EDTA plasma(n=5) |
84-101% |
83-101% |
88-93% |
|
heparin plasma(n=5) |
83-96% |
80-94% |
87-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
