FineTest
SKU:EH1815
Human WNT5B(Protein Wnt-5b) ELISA Kit
Human WNT5B(Protein Wnt-5b) ELISA Kit
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Product Name
Human WNT5B(Protein Wnt-5b) ELISA Kit
Alias
WNT5B ELISA Kit, Protein Wnt-5b ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
WNT5B ELISA Kit allows for the in vitro quantitative determination of WNT5B concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.781-50ng/ml
Sensitivity
< 0.469ng/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of WNT5B and the recovery rates were calculated by comparing the measured value to the expected amount of WNT5B in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
92-104 |
98 |
|
EDTA plasma(n=5) |
87-104 |
95 |
|
heparin plasma(n=5) |
85-103 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of WNT5B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-105% |
90-102% |
86-103% |
|
EDTA plasma(n=5) |
85-94% |
85-101% |
84-101% |
|
heparin plasma(n=5) |
81-99% |
80-100% |
80-95% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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