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SKU:EH1603
Human SIK1(Serine/threonine-protein kinase SIK1) ELISA Kit
Human SIK1(Serine/threonine-protein kinase SIK1) ELISA Kit
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SIK1|Serine|threonine-protein kinase SIK1|SIK|SIK-1|SNF1LK|Salt-inducible protein kinase 1|Serine|threonine-protein kinase SNF1-like kinase 1|Serine|threonine-protein kinase SNF1LK
Product Name
Human SIK1(Serine/threonine-protein kinase SIK1) ELISA Kit
Alias
SIK1 ELISA Kit, Serine ELISA Kit, threonine-protein kinase SIK1 ELISA Kit, SIK ELISA Kit, SIK-1 ELISA Kit, SNF1LK ELISA Kit, Salt-inducible protein kinase 1 ELISA Kit, Serine ELISA Kit, threonine-protein kinase SNF1-like kinase 1 ELISA Kit, Serine ELISA Kit, threonine-protein kinase SNF1LK ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
SIK1 ELISA Kit allows for the in vitro quantitative determination of SIK1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of SIK1 and the recovery rates were calculated by comparing the measured value to the expected amount of SIK1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-103 |
94 |
|
EDTA plasma(n=5) |
88-104 |
98 |
|
heparin plasma(n=5) |
90-103 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SIK1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-103% |
92-103% |
88-103% |
|
EDTA plasma(n=5) |
83-93% |
88-100% |
82-101% |
|
heparin plasma(n=5) |
80-100% |
85-100% |
81-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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