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SKU:EH1594

Human PIK3CA(Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform) ELISA Kit

Human PIK3CA(Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform) ELISA Kit

Regular price $450.00 USD
Regular price Sale price $450.00 USD
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size
Sensitivity
Range

PIK3CA|Phosphatidylinositol-4|5-bisphosphate 3-kinase catalytic subunit alpha isoform|PI3-kinase subunit alpha|PI3K-alpha|PI3Kalpha|PtdIns-3-kinase subunit alpha|Phosphatidylinositol-4|5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha|PtdIns-3-kinase subunit p110-alpha|p110alpha|Phosphoinositide-3-kinase catalytic alpha polypeptide

Product Name

Human PIK3CA(Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform) ELISA Kit

Alias

PIK3CA ELISA Kit, Phosphatidylinositol-4 ELISA Kit, 5-bisphosphate 3-kinase catalytic subunit alpha isoform ELISA Kit, PI3-kinase subunit alpha ELISA Kit, PI3K-alpha ELISA Kit, PI3Kalpha ELISA Kit, PtdIns-3-kinase subunit alpha ELISA Kit, Phosphatidylinositol-4 ELISA Kit, 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha ELISA Kit, PtdIns-3-kinase subunit p110-alpha ELISA Kit, p110alpha ELISA Kit, Phosphoinositide-3-kinase catalytic alpha polypeptide ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

PIK3CA ELISA Kit allows for the in vitro quantitative determination of PIK3CA concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Species

Human

UniProt ID

P42336

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of PIK3CA and the recovery rates were calculated by comparing the measured value to the expected amount of PIK3CA in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

88-103

95

EDTA plasma(n=5)

86-103

95

heparin plasma(n=5)

88-105

97

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PIK3CA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

88-104%

92-104%

86-99%

EDTA plasma(n=5)

83-94%

83-99%

82-88%

heparin plasma(n=5)

84-98%

80-99%

85-100%

CV(%)

Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note

For Research Use Only

 

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