FineTest
SKU:EH1570
Human WNT1(Proto-oncogene Wnt-1) ELISA Kit
Human WNT1(Proto-oncogene Wnt-1) ELISA Kit
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WNT1|Proto-oncogene Wnt-1|Proto-oncogene Int-1 homolog|INT1|OI15|BMND16
Product Name
Human WNT1(Proto-oncogene Wnt-1) ELISA Kit
Alias
WNT1 ELISA Kit, Proto-oncogene Wnt-1 ELISA Kit, Proto-oncogene Int-1 homolog ELISA Kit, INT1 ELISA Kit, OI15 ELISA Kit, BMND16 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
WNT1 ELISA Kit allows for the in vitro quantitative determination of WNT1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
12.5-800pg/ml
Sensitivity
< 7.5pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of WNT1 and the recovery rates were calculated by comparing the measured value to the expected amount of WNT1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-101 |
95 |
|
EDTA plasma(n=5) |
89-97 |
92 |
|
heparin plasma(n=5) |
87-103 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of WNT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-104% |
90-105% |
91-105% |
|
EDTA plasma(n=5) |
86-98% |
86-98% |
83-99% |
|
heparin plasma(n=5) |
80-100% |
80-96% |
84-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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