FineTest
SKU:EH1545
Human DBT(Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial) ELISA Kit
Human DBT(Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial) ELISA Kit
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DBT|BCATE2|BCKAD-E2|BCKADE2|E2|E2B|Dihydrolipoyllysine-residue|2-methylpropanoyltransferase|Dihydrolipoamide branched chain transacylase|Branched-chain alpha-keto acid dehydrogenase complex component E2|Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex
Product Name
Human DBT(Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial) ELISA Kit
Alias
DBT ELISA Kit, BCATE2 ELISA Kit, BCKAD-E2 ELISA Kit, BCKADE2 ELISA Kit, E2 ELISA Kit, E2B ELISA Kit, Dihydrolipoyllysine-residue ELISA Kit, 2-methylpropanoyltransferase ELISA Kit, Dihydrolipoamide branched chain transacylase ELISA Kit, Branched-chain alpha-keto acid dehydrogenase complex component E2 ELISA Kit, Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
DBT ELISA Kit allows for the in vitro quantitative determination of DBT concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of DBT and the recovery rates were calculated by comparing the measured value to the expected amount of DBT in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-102 |
91 |
|
EDTA plasma(n=5) |
91-97 |
95 |
|
heparin plasma(n=5) |
91-104 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DBT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-100% |
89-102% |
87-95% |
|
EDTA plasma(n=5) |
82-100% |
86-100% |
86-93% |
|
heparin plasma(n=5) |
87-100% |
84-98% |
84-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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