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SKU:EH1545

Human DBT(Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial) ELISA Kit

Human DBT(Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial) ELISA Kit

Regular price $450.00 USD
Regular price Sale price $450.00 USD
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size
Sensitivity
Range

DBT|BCATE2|BCKAD-E2|BCKADE2|E2|E2B|Dihydrolipoyllysine-residue|2-methylpropanoyltransferase|Dihydrolipoamide branched chain transacylase|Branched-chain alpha-keto acid dehydrogenase complex component E2|Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex

Product Name

Human DBT(Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial) ELISA Kit

Alias

DBT ELISA Kit, BCATE2 ELISA Kit, BCKAD-E2 ELISA Kit, BCKADE2 ELISA Kit, E2 ELISA Kit, E2B ELISA Kit, Dihydrolipoyllysine-residue ELISA Kit, 2-methylpropanoyltransferase ELISA Kit, Dihydrolipoamide branched chain transacylase ELISA Kit, Branched-chain alpha-keto acid dehydrogenase complex component E2 ELISA Kit, Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

DBT ELISA Kit allows for the in vitro quantitative determination of DBT concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Species

Human

UniProt ID

P11182

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of DBT and the recovery rates were calculated by comparing the measured value to the expected amount of DBT in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

86-102

91

EDTA plasma(n=5)

91-97

95

heparin plasma(n=5)

91-104

98

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DBT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

87-100%

89-102%

87-95%

EDTA plasma(n=5)

82-100%

86-100%

86-93%

heparin plasma(n=5)

87-100%

84-98%

84-99%

CV(%)

Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note

For Research Use Only

 

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