FineTest
SKU:EH1354
Human TOP2B(DNA topoisomerase 2-beta) ELISA Kit
Human TOP2B(DNA topoisomerase 2-beta) ELISA Kit
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TOP2B|DNA topoisomerase 2-beta|DNA topoisomerase II|beta isozyme|TOPIIB|top2beta
Product Name
Human TOP2B(DNA topoisomerase 2-beta) ELISA Kit
Alias
TOP2B ELISA Kit, DNA topoisomerase 2-beta ELISA Kit, DNA topoisomerase II ELISA Kit, beta isozyme ELISA Kit, TOPIIB ELISA Kit, top2beta ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
TOP2B ELISA Kit allows for the in vitro quantitative determination of TOP2B concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of TOP2B and the recovery rates were calculated by comparing the measured value to the expected amount of TOP2B in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
91-105 |
98 |
|
EDTA plasma(n=5) |
85-103 |
97 |
|
heparin plasma(n=5) |
86-104 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TOP2B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-101% |
88-96% |
88-103% |
|
EDTA plasma(n=5) |
83-100% |
83-100% |
85-97% |
|
heparin plasma(n=5) |
87-98% |
87-100% |
80-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
