FineTest
SKU:EH1353
Human FLG(Filaggrin) ELISA Kit
Human FLG(Filaggrin) ELISA Kit
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FLG|Filaggrin|ATOD2
Product Name
Human FLG(Filaggrin) ELISA Kit
Alias
FLG ELISA Kit, Filaggrin ELISA Kit, ATOD2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
FLG ELISA Kit allows for the in vitro quantitative determination of FLG concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of FLG and the recovery rates were calculated by comparing the measured value to the expected amount of FLG in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-105 |
98 |
|
EDTA plasma(n=5) |
88-104 |
95 |
|
heparin plasma(n=5) |
89-105 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FLG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
92-103% |
87-97% |
85-104% |
|
EDTA plasma(n=5) |
85-97% |
84-99% |
85-97% |
|
heparin plasma(n=5) |
82-98% |
80-97% |
85-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
