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SKU:EH1339
Human IDH2(IsocitRate dehydrogenase [NADP], mitochondrial) ELISA Kit
Human IDH2(IsocitRate dehydrogenase [NADP], mitochondrial) ELISA Kit
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IDH2|IsocitRate dehydrogenase [NADP]|mitochondrial|ICD-M|IDH|IDHM|IDP|mNADP-IDH|Oxalosuccinate decarboxylase|NADP|+-specific ICDH|ICD-M
Product Name
Human IDH2(IsocitRate dehydrogenase [NADP], mitochondrial) ELISA Kit
Alias
IDH2 ELISA Kit, IsocitRate dehydrogenase [NADP] ELISA Kit, mitochondrial ELISA Kit, ICD-M ELISA Kit, IDH ELISA Kit, IDHM ELISA Kit, IDP ELISA Kit, mNADP-IDH ELISA Kit, Oxalosuccinate decarboxylase ELISA Kit, NADP ELISA Kit, +-specific ICDH ELISA Kit, ICD-M ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IDH2 ELISA Kit allows for the in vitro quantitative determination of IDH2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IDH2 and the recovery rates were calculated by comparing the measured value to the expected amount of IDH2 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-100 |
94 |
|
EDTA plasma(n=5) |
85-102 |
94 |
|
heparin plasma(n=5) |
89-101 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IDH2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-103% |
86-104% |
93-105% |
|
EDTA plasma(n=5) |
85-101% |
86-96% |
92-100% |
|
heparin plasma(n=5) |
81-96% |
82-92% |
86-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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