FineTest
SKU:EH1321
Human WT1(Wilms tumor protein) ELISA Kit
Human WT1(Wilms tumor protein) ELISA Kit
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WT1|Wilms tumor protein|GUD|WAGR|WIT-2|WT33|AWT1|NPHS4GUD|WAGR|Wilms tumor 1
Product Name
Human WT1(Wilms tumor protein) ELISA Kit
Alias
WT1 ELISA Kit, Wilms tumor protein ELISA Kit, GUD ELISA Kit, WAGR ELISA Kit, WIT-2 ELISA Kit, WT33 ELISA Kit, AWT1 ELISA Kit, NPHS4GUD ELISA Kit, WAGR ELISA Kit, Wilms tumor 1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
WT1 ELISA Kit allows for the in vitro quantitative determination of WT1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of WT1 and the recovery rates were calculated by comparing the measured value to the expected amount of WT1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-105 |
97 |
|
EDTA plasma(n=5) |
88-103 |
96 |
|
heparin plasma(n=5) |
86-101 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of WT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-104% |
89-103% |
87-104% |
|
EDTA plasma(n=5) |
83-101% |
85-101% |
83-100% |
|
heparin plasma(n=5) |
86-98% |
81-100% |
86-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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