FineTest
SKU:EH1295
Human SIRT5(NAD-dependent deacetylase sirtuin-5) ELISA Kit
Human SIRT5(NAD-dependent deacetylase sirtuin-5) ELISA Kit
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SIRT5|NAD-dependent deacetylase sirtuin-5|SIR2L5|SIRT5|silent mating type information regulation 2|S.cerevisiae|homolog 5|sir2-like 5|SIR2-like protein 5|sirtuin 5|sirtuin type 5|Regulatory protein SIR2 homolog 5
Product Name
Human SIRT5(NAD-dependent deacetylase sirtuin-5) ELISA Kit
Alias
SIRT5 ELISA Kit, NAD-dependent deacetylase sirtuin-5 ELISA Kit, SIR2L5 ELISA Kit, SIRT5 ELISA Kit, silent mating type information regulation 2 ELISA Kit, S.cerevisiae ELISA Kit, homolog 5 ELISA Kit, sir2-like 5 ELISA Kit, SIR2-like protein 5 ELISA Kit, sirtuin 5 ELISA Kit, sirtuin type 5 ELISA Kit, Regulatory protein SIR2 homolog 5 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
SIRT5 ELISA Kit allows for the in vitro quantitative determination of SIRT5 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of SIRT5 and the recovery rates were calculated by comparing the measured value to the expected amount of SIRT5 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-103 |
95 |
|
EDTA plasma(n=5) |
85-104 |
94 |
|
heparin plasma(n=5) |
85-104 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SIRT5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-104% |
90-105% |
88-101% |
|
EDTA plasma(n=5) |
86-101% |
82-97% |
94-98% |
|
heparin plasma(n=5) |
84-100% |
89-98% |
82-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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