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SKU:EH1290
Human PRAME(Melanoma antigen preferentially expressed in tumors) ELISA Kit
Human PRAME(Melanoma antigen preferentially expressed in tumors) ELISA Kit
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PRAME|Melanoma antigen preferentially expressed in tumors|MAPE|OIP4|OIP-4|CT130|Cancer|testis antigen 130|Opa-interacting protein 4|Preferentially expressed antigen of melanoma
Product Name
Human PRAME(Melanoma antigen preferentially expressed in tumors) ELISA Kit
Alias
PRAME ELISA Kit, Melanoma antigen preferentially expressed in tumors ELISA Kit, MAPE ELISA Kit, OIP4 ELISA Kit, OIP-4 ELISA Kit, CT130 ELISA Kit, Cancer ELISA Kit, testis antigen 130 ELISA Kit, Opa-interacting protein 4 ELISA Kit, Preferentially expressed antigen of melanoma ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PRAME ELISA Kit allows for the in vitro quantitative determination of PRAME concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PRAME and the recovery rates were calculated by comparing the measured value to the expected amount of PRAME in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-102 |
94 |
|
EDTA plasma(n=5) |
87-101 |
95 |
|
heparin plasma(n=5) |
90-103 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PRAME and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-101% |
89-104% |
87-105% |
|
EDTA plasma(n=5) |
83-98% |
82-96% |
83-97% |
|
heparin plasma(n=5) |
85-99% |
87-97% |
82-95% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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