FineTest
SKU:EH1279
Human C4B(Complement C4-B) ELISA Kit
Human C4B(Complement C4-B) ELISA Kit
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C4B|Complement C4-B|Basic complement C4|C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3|C4-B|CPAMD3|C4|Chido Blood Group|Basic C4|CO4|CPAMD3
Product Name
Human C4B(Complement C4-B) ELISA Kit
Alias
C4B ELISA Kit, Complement C4-B ELISA Kit, Basic complement C4 ELISA Kit, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 ELISA Kit, C4-B ELISA Kit, CPAMD3 ELISA Kit, C4 ELISA Kit, Chido Blood Group ELISA Kit, Basic C4 ELISA Kit, CO4 ELISA Kit, CPAMD3 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
C4B ELISA Kit allows for the in vitro quantitative determination of C4B concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
4.688-300ng/ml
Sensitivity
< 2.813ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of C4B and the recovery rates were calculated by comparing the measured value to the expected amount of C4B in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-103 |
97 |
|
EDTA plasma(n=5) |
89-103 |
95 |
|
heparin plasma(n=5) |
88-101 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C4B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-103% |
87-103% |
87-99% |
|
EDTA plasma(n=5) |
82-97% |
85-99% |
90-97% |
|
heparin plasma(n=5) |
81-100% |
82-99% |
88-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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