FineTest
SKU:EH1251
Human ACTN3(Alpha-actinin-3) ELISA Kit
Human ACTN3(Alpha-actinin-3) ELISA Kit
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ACTN3|Actinin Alpha 3|alpha-actinin skeletal muscle isoform 3|F-actin cross-linking protein
Product Name
Human ACTN3(Alpha-actinin-3) ELISA Kit
Alias
ACTN3 ELISA Kit, Actinin Alpha 3 ELISA Kit, alpha-actinin skeletal muscle isoform 3 ELISA Kit, F-actin cross-linking protein ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
ACTN3 ELISA Kit allows for the in vitro quantitative determination of ACTN3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of ACTN3 and the recovery rates were calculated by comparing the measured value to the expected amount of ACTN3 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-101 |
97 |
|
EDTA plasma(n=5) |
89-96 |
94 |
|
heparin plasma(n=5) |
87-105 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACTN3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-101% |
86-104% |
94-105% |
|
EDTA plasma(n=5) |
86-101% |
82-98% |
82-96% |
|
heparin plasma(n=5) |
82-98% |
82-95% |
81-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
