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SKU:EH1205
Human NEFL(Neurofilament light polypeptide) ELISA Kit
Human NEFL(Neurofilament light polypeptide) ELISA Kit
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NEFL|Neurofilament light polypeptide|NF-L|68 kDa neurofilament protein|Neurofilament triplet L protein
Product Name
Human NEFL(Neurofilament light polypeptide) ELISA Kit
Alias
NEFL ELISA Kit, Neurofilament light polypeptide ELISA Kit, NF-L ELISA Kit, 68 kDa neurofilament protein ELISA Kit, Neurofilament triplet L protein ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
NEFL ELISA Kit allows for the in vitro quantitative determination of NEFL concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of NEFL and the recovery rates were calculated by comparing the measured value to the expected amount of NEFL in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-103 |
94 |
|
EDTA plasma(n=5) |
87-100 |
93 |
|
heparin plasma(n=5) |
90-98 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NEFL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
89-100% |
90-105% |
86-101% |
|
EDTA plasma(n=5) |
82-98% |
88-100% |
85-100% |
|
heparin plasma(n=5) |
81-100% |
81-98% |
80-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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