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SKU:EH1191

Human CYP7A1(Cholesterol 7-alpha-monooxygenase) ELISA Kit

Human CYP7A1(Cholesterol 7-alpha-monooxygenase) ELISA Kit

Regular price $450.00 USD
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size
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CYP7A1|Cytochrome P450 7A1|Cholesterol 7-alpha-monooxygenase|CYPVII|Cholesterol 7-alpha-hydroxylase Introducing the Human Cholesterol 7-alpha-Monooxygenase, CYP7A1 ELISA Kit, a cutting-edge solution designed to facilitate accurate and efficient measurement of CYP7A1 levels in human cholesterol metabolism. This ELISA kit is meticulously crafted to meet the highest standards of quality and reliability, ensuring precise quantification of CYP7A1 enzyme activity.

The Human Cholesterol 7-alpha-Monooxygenase, CYP7A1 ELISA Kit employs a highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) technique, enabling researchers and clinicians to detect and quantify CYP7A1 levels with utmost precision. This advanced kit offers a comprehensive and user-friendly platform for the assessment of CYP7A1, a crucial enzyme involved in the conversion of cholesterol to bile acids.

With its state-of-the-art components and optimized protocols, this ELISA kit guarantees exceptional accuracy and reproducibility, allowing for reliable and consistent results. The kit includes all necessary reagents and materials, meticulously selected and rigorously tested to ensure optimal performance and minimal interference.

The Human Cholesterol 7-alpha-Monooxygenase, CYP7A1 ELISA Kit is designed to streamline and expedite the measurement process, offering a rapid and efficient workflow. Its user-friendly nature and clear instructions make it accessible to both seasoned researchers and novices in the field, facilitating seamless integration into various laboratory settings.

This ELISA kit is an indispensable tool for studying cholesterol metabolism and its associated disorders, providing valuable insights into the intricate mechanisms underlying cholesterol homeostasis. Its high sensitivity and accuracy make it an ideal choice for research, drug discovery, and clinical applications, enabling researchers and healthcare professionals to make informed decisions and advancements in the field.

In summary, the Human Cholesterol 7-alpha-Monooxygenase, CYP7A1 ELISA Kit is a state-of-the-art solution that empowers researchers and clinicians with a reliable and efficient method for quantifying CYP7A1 levels in human cholesterol metabolism. With its exceptional accuracy, sensitivity, and user-friendly design, this ELISA kit is poised to revolutionize the study and understanding of cholesterol metabolism, paving the way for groundbreaking discoveries and advancements in the field.

Product Name

Human CYP7A1(Cholesterol 7-alpha-monooxygenase) ELISA Kit

Alias

CYP7A1 ELISA KitCytochrome P450 7A1 ELISA KitCholesterol 7-alpha-monooxygenase ELISA KitCYPVII ELISA KitCholesterol 7-alpha-hydroxylase ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

CYP7A1 ELISA Kit allows for the in vitro quantitative determination of CYP7A1 concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Species

Human

UniProt ID

P22680

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of CYP7A1 and the recovery rates were calculated by comparing the measured value to the expected amount of CYP7A1 in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

90-99

94

EDTA plasma(n=5)

95-103

98

heparin plasma(n=5)

85-97

91

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CYP7A1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

90-104%

90-99%

87-91%

EDTA plasma(n=5)

82-94%

85-97%

83-95%

heparin plasma(n=5)

82-100%

81-93%

84-94%

CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

 

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