FineTest
SKU:EH1166
Human VIM(Vimentin) ELISA Kit
Human VIM(Vimentin) ELISA Kit
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VIM|Vimentin
Product Name
Human VIM(Vimentin) ELISA Kit
Alias
VIM ELISA Kit, Vimentin ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
VIM ELISA Kit allows for the in vitro quantitative determination of VIM concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
7.813-500ng/ml
Sensitivity
< 4.688ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of VIM and the recovery rates were calculated by comparing the measured value to the expected amount of VIM in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
91-101 |
97 |
|
EDTA plasma(n=5) |
89-104 |
95 |
|
heparin plasma(n=5) |
85-103 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VIM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-95% |
91-101% |
85-100% |
|
EDTA plasma(n=5) |
87-101% |
84-101% |
89-101% |
|
heparin plasma(n=5) |
88-98% |
80-100% |
82-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
