Human CYP27B1(25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial) ELISA Kit

CYP27B1|VD3 1A hydroxylase|Cytochrome P450VD1-alpha|Cytochrome P450C1 alpha|Cytochrome P450 subfamily XXVIIB polypeptide 1|Calcidiol 1-monooxygenase|Cytochrome p450 27B1|25-OHD-1 alpha-hydroxylase|25-hydroxyvitamin D|3 1-alpha-hydroxylase

Product Name

Human CYP27B1(25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial) ELISA Kit

Alias

CYP27B1 ELISA Kit, VD3 1A hydroxylase ELISA Kit, Cytochrome P450VD1-alpha ELISA Kit, Cytochrome P450C1 alpha ELISA Kit, Cytochrome P450 subfamily XXVIIB polypeptide 1 ELISA Kit, Calcidiol 1-monooxygenase ELISA Kit, Cytochrome p450 27B1 ELISA Kit, 25-OHD-1 alpha-hydroxylase ELISA Kit, 25-hydroxyvitamin D ELISA Kit, 3 1-alpha-hydroxylase ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

CYP27B1 ELISA Kit allows for the in vitro quantitative determination of CYP27B1 concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.781-50ng/ml

Sensitivity

< 0.469ng/ml

Species

Human

UniProt ID

O15528

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of CYP27B1 and the recovery rates were calculated by comparing the measured value to the expected amount of CYP27B1 in samples.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CYP27B1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only