FineTest
SKU:EH1146
Human BECN1(Beclin-1) ELISA Kit
Human BECN1(Beclin-1) ELISA Kit
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BECN1|Beclin 1|ATG6|GT197|VPS30|ATG6 autophagy related 6 homolog|beclin 1|autophagy related|beclin-1|Coiled-coil myosin-like BCL2-interacting protein|Protein GT197
Product Name
Human BECN1(Beclin-1) ELISA Kit
Alias
BECN1 ELISA Kit, Beclin 1 ELISA Kit, ATG6 ELISA Kit, GT197 ELISA Kit, VPS30 ELISA Kit, ATG6 autophagy related 6 homolog ELISA Kit, beclin 1 ELISA Kit, autophagy related ELISA Kit, beclin-1 ELISA Kit, Coiled-coil myosin-like BCL2-interacting protein ELISA Kit, Protein GT197 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
BECN1 ELISA Kit allows for the in vitro quantitative determination of BECN1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of BECN1 and the recovery rates were calculated by comparing the measured value to the expected amount of BECN1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
95-102 |
99 |
|
EDTA plasma(n=5) |
85-105 |
94 |
|
heparin plasma(n=5) |
86-105 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BECN1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-104% |
90-103% |
88-104% |
|
EDTA plasma(n=5) |
86-94% |
87-97% |
84-99% |
|
heparin plasma(n=5) |
84-99% |
89-99% |
86-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
