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SKU:EH1106
Human UBE2A(Ubiquitin-conjugating enzyme E2 A) ELISA Kit
Human UBE2A(Ubiquitin-conjugating enzyme E2 A) ELISA Kit
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UBE2A|RAD6A|UBE2A|HHR6A|HR6A|RAD6 homolog A|UBC2|Ubiquitin carrier protein A|ubiquitin-conjugating enzyme E2 A|Ubiquitin-protein ligase A
Product Name
Human UBE2A(Ubiquitin-conjugating enzyme E2 A) ELISA Kit
Alias
UBE2A ELISA Kit, RAD6A ELISA Kit, UBE2A ELISA Kit, HHR6A ELISA Kit, HR6A ELISA Kit, RAD6 homolog A ELISA Kit, UBC2 ELISA Kit, Ubiquitin carrier protein A ELISA Kit, ubiquitin-conjugating enzyme E2 A ELISA Kit, Ubiquitin-protein ligase A ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
UBE2A ELISA Kit allows for the in vitro quantitative determination of UBE2A concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of UBE2A and the recovery rates were calculated by comparing the measured value to the expected amount of UBE2A in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-105 |
93 |
|
EDTA plasma(n=5) |
88-102 |
94 |
|
heparin plasma(n=5) |
86-98 |
91 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of UBE2A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-105% |
88-98% |
95-105% |
|
EDTA plasma(n=5) |
82-101% |
88-96% |
85-101% |
|
heparin plasma(n=5) |
85-96% |
81-97% |
85-93% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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