FineTest
SKU:EH1085
Human PCK1(Phosphoenolpyruvate carboxykinase, cytosolic [GTP]) ELISA Kit
Human PCK1(Phosphoenolpyruvate carboxykinase, cytosolic [GTP]) ELISA Kit
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PCK1|Phosphoenolpyruvate Carboxykinase 1|PEPCK1|PEPCK-C|PEPCKC|PEPCK-CPEP carboxykinase|phosphoenolpyruvate carboxykinase 1|soluble|phosphoenolpyruvate carboxykinase|cytosolic|phosphoenolpyruvate carboxykinase|cytosolic [GTP]|Phosphoenolpyruvate carboxylase
Product Name
Human PCK1(Phosphoenolpyruvate carboxykinase, cytosolic [GTP]) ELISA Kit
Alias
PCK1 ELISA Kit, Phosphoenolpyruvate Carboxykinase 1 ELISA Kit, PEPCK1 ELISA Kit, PEPCK-C ELISA Kit, PEPCKC ELISA Kit, PEPCK-CPEP carboxykinase ELISA Kit, phosphoenolpyruvate carboxykinase 1 ELISA Kit, soluble ELISA Kit, phosphoenolpyruvate carboxykinase ELISA Kit, cytosolic ELISA Kit, phosphoenolpyruvate carboxykinase ELISA Kit, cytosolic [GTP] ELISA Kit, Phosphoenolpyruvate carboxylase ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PCK1 ELISA Kit allows for the in vitro quantitative determination of PCK1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PCK1 and the recovery rates were calculated by comparing the measured value to the expected amount of PCK1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-105 |
97 |
|
EDTA plasma(n=5) |
85-102 |
97 |
|
heparin plasma(n=5) |
88-98 |
91 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PCK1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
95-104% |
91-100% |
86-98% |
|
EDTA plasma(n=5) |
82-99% |
82-99% |
83-98% |
|
heparin plasma(n=5) |
82-97% |
87-100% |
81-95% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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