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SKU:EH1048
Human SERPINF1(Pigment epithelium-derived factor) ELISA Kit
Human SERPINF1(Pigment epithelium-derived factor) ELISA Kit
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PEDF|Pigment Epithelium Derived Factor|Serpin F1|Pigment epithelium-derived factor|Cell prolifeRation-inducing gene 35 protein|EPC-1
Product Name
Human SERPINF1(Pigment epithelium-derived factor) ELISA Kit
Alias
PEDF ELISA Kit, Pigment Epithelium Derived Factor ELISA Kit, Serpin F1 ELISA Kit, Pigment epithelium-derived factor ELISA Kit, Cell prolifeRation-inducing gene 35 protein ELISA Kit, EPC-1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
SERPINF1 ELISA Kit allows for the in vitro quantitative determination of SERPINF1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of SERPINF1 and the recovery rates were calculated by comparing the measured value to the expected amount of SERPINF1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-105 |
96 |
|
EDTA plasma(n=5) |
86-105 |
95 |
|
heparin plasma(n=5) |
87-105 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SERPINF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-103% |
85-103% |
88-104% |
|
EDTA plasma(n=5) |
87-99% |
84-97% |
84-97% |
|
heparin plasma(n=5) |
80-93% |
85-96% |
83-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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