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SKU:EH1012
Human F12(Coagulation factor XII) ELISA Kit
Human F12(Coagulation factor XII) ELISA Kit
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F12|Coagulation factor XII|HAEX|Hageman factor|HAE3|HAF
Product Name
Human F12(Coagulation factor XII) ELISA Kit
Alias
F12 ELISA Kit, Coagulation factor XII ELISA Kit, HAEX ELISA Kit, Hageman factor ELISA Kit, HAE3 ELISA Kit, HAF ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
F12 ELISA Kit allows for the in vitro quantitative determination of F12 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
3.125-200ng/ml
Sensitivity
< 1.875ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of F12 and the recovery rates were calculated by comparing the measured value to the expected amount of F12 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
91-100 |
95 |
|
EDTA plasma(n=5) |
89-105 |
99 |
|
heparin plasma(n=5) |
86-98 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-95% |
87-101% |
85-102% |
|
EDTA plasma(n=5) |
86-99% |
82-92% |
93-101% |
|
heparin plasma(n=5) |
82-99% |
83-99% |
81-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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