FineTest
SKU:EH0959
Human COL2A1(Collagen alpha-1(II) chain) ELISA Kit
Human COL2A1(Collagen alpha-1(II) chain) ELISA Kit
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COL2α1|COL2A1|ANFH|AOM|COL11A3|SEDC|Chondrocalcin|Alpha-1 type II collagen|cartilage collagen|Collagen 2|collagen alpha-1|II chain|collagen II|alpha-1 polypeptide|collagen type 2|Collagen-2
Product Name
Human COL2A1(Collagen alpha-1(II) chain) ELISA Kit
Alias
COL2α1 ELISA Kit, COL2A1 ELISA Kit, ANFH ELISA Kit, AOM ELISA Kit, COL11A3 ELISA Kit, SEDC ELISA Kit, Chondrocalcin ELISA Kit, Alpha-1 type II collagen ELISA Kit, cartilage collagen ELISA Kit, Collagen 2 ELISA Kit, collagen alpha-1 ELISA Kit, II chain ELISA Kit, collagen II ELISA Kit, alpha-1 polypeptide ELISA Kit, collagen type 2 ELISA Kit, Collagen-2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
COL2A1 chain) ELISA Kit allows for the in vitro quantitative determination of COL2A1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.625-40ng/ml
Sensitivity
< 0.375ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of COL2A1 and the recovery rates were calculated by comparing the measured value to the expected amount of COL2A1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-103 |
94 |
|
EDTA plasma(n=5) |
86-101 |
96 |
|
heparin plasma(n=5) |
85-105 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of COL2A1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-104% |
89-98% |
85-103% |
|
EDTA plasma(n=5) |
82-100% |
82-101% |
84-99% |
|
heparin plasma(n=5) |
85-99% |
81-97% |
80-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
