FineTest
SKU:EH0958
Human COL1A1(Collagen Type I Alpha 1) ELISA Kit
Human COL1A1(Collagen Type I Alpha 1) ELISA Kit
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COL1α1|COL1A1|Collagen Type I Alpha 1|Alpha-1 type I collagen|OI4|Collagen 1|collagen alpha 1 chain type I|Collagen1|Collagen-1|OI4|pro-alpha-1 collagen type 1
Product Name
Human COL1A1(Collagen Type I Alpha 1) ELISA Kit
Alias
COL1α1 ELISA Kit, COL1A1 ELISA Kit, Collagen Type I Alpha 1 ELISA Kit, Alpha-1 type I collagen ELISA Kit, OI4 ELISA Kit, Collagen 1 ELISA Kit, collagen alpha 1 chain type I ELISA Kit, Collagen1 ELISA Kit, Collagen-1 ELISA Kit, OI4 ELISA Kit, pro-alpha-1 collagen type 1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
COL1A1 ELISA Kit allows for the in vitro quantitative determination of COL1A1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of COL1A1 and the recovery rates were calculated by comparing the measured value to the expected amount of COL1A1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-92 |
91 |
|
EDTA plasma(n=5) |
90-105 |
96 |
|
heparin plasma(n=5) |
86-98 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of COL1A1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-102% |
88-103% |
89-105% |
|
EDTA plasma(n=5) |
85-101% |
87-95% |
85-94% |
|
heparin plasma(n=5) |
85-96% |
86-94% |
85-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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