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SKU:EH0947
Human MITF(Microphthalmia-associated transcription factor) ELISA Kit
Human MITF(Microphthalmia-associated transcription factor) ELISA Kit
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MITF|BHLHE32|Microphthalmia-associated transcription factor|MI|WS2A|Waardenburg syndrome|type 2A|Class E basic helix-loop-helix protein 32
Product Name
Human MITF(Microphthalmia-associated transcription factor) ELISA Kit
Alias
MITF ELISA Kit, BHLHE32 ELISA Kit, Microphthalmia-associated transcription factor ELISA Kit, MI ELISA Kit, WS2A ELISA Kit, Waardenburg syndrome ELISA Kit, type 2A ELISA Kit, Class E basic helix-loop-helix protein 32 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
MITF ELISA Kit allows for the in vitro quantitative determination of MITF concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.625-40ng/ml
Sensitivity
< 0.375ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of MITF and the recovery rates were calculated by comparing the measured value to the expected amount of MITF in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-103 |
96 |
|
EDTA plasma(n=5) |
85-100 |
93 |
|
heparin plasma(n=5) |
88-104 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MITF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-98% |
85-103% |
90-103% |
|
EDTA plasma(n=5) |
84-98% |
82-98% |
87-95% |
|
heparin plasma(n=5) |
81-91% |
82-96% |
83-95% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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