FineTest
SKU:EH0909
Human SESN1(Sestrin-1) ELISA Kit
Human SESN1(Sestrin-1) ELISA Kit
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SESN1|Sestrin-1|PA26|SEST1|p53-regulated protein PA26|
Product Name
Human SESN1(Sestrin-1) ELISA Kit
Alias
SESN1 ELISA Kit, Sestrin-1 ELISA Kit, PA26 ELISA Kit, SEST1 ELISA Kit, p53-regulated protein PA26 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
SESN1 ELISA Kit allows for the in vitro quantitative determination of SESN1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.625-40ng/ml
Sensitivity
< 0.375ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of SESN1 and the recovery rates were calculated by comparing the measured value to the expected amount of SESN1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-103 |
93 |
|
EDTA plasma(n=5) |
86-98 |
92 |
|
heparin plasma(n=5) |
90-104 |
99 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SESN1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-103% |
98-103% |
88-104% |
|
EDTA plasma(n=5) |
82-100% |
85-100% |
88-97% |
|
heparin plasma(n=5) |
88-99% |
85-99% |
80-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
