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SKU:EH0904

Human PDE5A(cGMP-specific 3',5'-cyclic phosphodiesterase) ELISA Kit

Human PDE5A(cGMP-specific 3',5'-cyclic phosphodiesterase) ELISA Kit

Regular price $450.00 USD
Regular price Sale price $450.00 USD
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size
Sensitivity
Range

PDE5A|cGMP-specific 3'|5'-cyclic phosphodiesterase||cGMP-binding cGMP-specific phosphodiesterase|CGB-PDE|PDE5

Product Name

Human PDE5A(cGMP-specific 3′,5′-cyclic phosphodiesterase) ELISA Kit

Alias

PDE5A ELISA KitcGMP-specific 3' ELISA Kit5'-cyclic phosphodiesterase ELISA KitcGMP-binding cGMP-specific phosphodiesterase ELISA KitCGB-PDE ELISA KitPDE5 ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

PDE5A ELISA Kit allows for the in vitro quantitative determination of PDE5A concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Species

Human

UniProt ID

O76074

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of PDE5A and the recovery rates were calculated by comparing the measured value to the expected amount of PDE5A in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

87-99

95

EDTA plasma(n=5)

88-99

94

heparin plasma(n=5)

85-105

95

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDE5A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

91-103%

85-100%

96-102%

EDTA plasma(n=5)

84-94%

83-91%

84-96%

heparin plasma(n=5)

83-94%

82-95%

91-100%

CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

 

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