FineTest
SKU:EH0884
Human HMGB1(High mobility group protein B1) ELISA Kit
Human HMGB1(High mobility group protein B1) ELISA Kit
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HMGB1|High mobility group protein B1|HMG-1|HMG1|HMG3|SBP-1|Amphoterin|high mobility group box 1|High mobility group protein 1|high-mobility group|nonhistone chromosomal protein 1|high-mobility group box 1|Sulfoglucuronyl carbohydRate binding protein
Product Name
Human HMGB1(High mobility group protein B1) ELISA Kit
Alias
HMGB1 ELISA Kit, High mobility group protein B1 ELISA Kit, HMG-1 ELISA Kit, HMG1 ELISA Kit, HMG3 ELISA Kit, SBP-1 ELISA Kit, Amphoterin ELISA Kit, high mobility group box 1 ELISA Kit, High mobility group protein 1 ELISA Kit, high-mobility group ELISA Kit, nonhistone chromosomal protein 1 ELISA Kit, high-mobility group box 1 ELISA Kit, Sulfoglucuronyl carbohydRate binding protein ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
HMGB1 ELISA Kit allows for the in vitro quantitative determination of HMGB1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of HMGB1 and the recovery rates were calculated by comparing the measured value to the expected amount of HMGB1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-103 |
98 |
|
EDTA plasma(n=5) |
88-100 |
95 |
|
heparin plasma(n=5) |
85-102 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HMGB1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
91-99% |
85-102% |
87-101% |
|
EDTA plasma(n=5) |
82-95% |
87-101% |
82-93% |
|
heparin plasma(n=5) |
82-100% |
85-99% |
85-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
