FineTest
SKU:EH0859
Human HEPH(Hephaestin) ELISA Kit
Human HEPH(Hephaestin) ELISA Kit
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HEPH|Hephaestin
Product Name
Human HEPH(Hephaestin) ELISA Kit
Alias
HEPH ELISA Kit, Hephaestin ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
HEPH ELISA Kit allows for the in vitro quantitative determination of HEPH concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of HEPH and the recovery rates were calculated by comparing the measured value to the expected amount of HEPH in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-105 |
97 |
|
EDTA plasma(n=5) |
85-103 |
94 |
|
heparin plasma(n=5) |
86-102 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HEPH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-103% |
89-104% |
88-104% |
|
EDTA plasma(n=5) |
82-96% |
85-97% |
82-95% |
|
heparin plasma(n=5) |
80-94% |
80-89% |
81-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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