FineTest
SKU:EH0836
Human SLC12A1(Solute carrier family 12 member 1) ELISA Kit
Human SLC12A1(Solute carrier family 12 member 1) ELISA Kit
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SLC12A1|Solute carrier family 12 member 1|NKCC2|Bumetanide-sensitive sodium-|potassium-chloride cotransporter 2|Kidney-specific Na-K-Cl symporter
Product Name
Human SLC12A1(Solute carrier family 12 member 1) ELISA Kit
Alias
SLC12A1 ELISA Kit, Solute carrier family 12 member 1 ELISA Kit, NKCC2 ELISA Kit, Bumetanide-sensitive sodium- ELISA Kit, potassium-chloride cotransporter 2 ELISA Kit, Kidney-specific Na-K-Cl symporter ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
SLC12A1 ELISA Kit allows for the in vitro quantitative determination of SLC12A1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of SLC12A1 and the recovery rates were calculated by comparing the measured value to the expected amount of SLC12A1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
93-101 |
97 |
|
EDTA plasma(n=5) |
88-101 |
94 |
|
heparin plasma(n=5) |
90-104 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SLC12A1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-101% |
88-101% |
88-100% |
|
EDTA plasma(n=5) |
85-93% |
84-101% |
82-101% |
|
heparin plasma(n=5) |
84-94% |
83-98% |
80-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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