FineTest
SKU:EH0806
Human HMGB3(High mobility group protein B3) ELISA Kit
Human HMGB3(High mobility group protein B3) ELISA Kit
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HMGB3|High mobility group protein B3|HMG2A|HMG4|High mobility group protein 2a|High mobility group protein 4|high-mobility group|nonhistone chromosomal protein 4|high-mobility group box 3|HMG-2a|HMG-4|non-histone chromosomal protein
Product Name
Human HMGB3(High mobility group protein B3) ELISA Kit
Alias
HMGB3 ELISA Kit, High mobility group protein B3 ELISA Kit, HMG2A ELISA Kit, HMG4 ELISA Kit, High mobility group protein 2a ELISA Kit, High mobility group protein 4 ELISA Kit, high-mobility group ELISA Kit, nonhistone chromosomal protein 4 ELISA Kit, high-mobility group box 3 ELISA Kit, HMG-2a ELISA Kit, HMG-4 ELISA Kit, non-histone chromosomal protein ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
HMGB3 ELISA Kit allows for the in vitro quantitative determination of HMGB3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of HMGB3 and the recovery rates were calculated by comparing the measured value to the expected amount of HMGB3 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-101 |
93 |
|
EDTA plasma(n=5) |
88-100 |
92 |
|
heparin plasma(n=5) |
85-101 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HMGB3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-104% |
85-103% |
87-105% |
|
EDTA plasma(n=5) |
82-101% |
85-101% |
83-97% |
|
heparin plasma(n=5) |
92-100% |
81-99% |
80-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
