FineTest
SKU:EH0798
Human ACAT1(Acetyl-CoA acetyltransferase, mitochondrial) ELISA Kit
Human ACAT1(Acetyl-CoA acetyltransferase, mitochondrial) ELISA Kit
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ACAT1|AACAT|MAT|Acetyl-CoA acetyltransferase|mitochondrial|Acetoacetyl-CoA thiolase|T2
Product Name
Human ACAT1(Acetyl-CoA acetyltransferase, mitochondrial) ELISA Kit
Alias
ACAT1 ELISA Kit, AACAT ELISA Kit, MAT ELISA Kit, Acetyl-CoA acetyltransferase ELISA Kit, mitochondrial ELISA Kit, Acetoacetyl-CoA thiolase ELISA Kit, T2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
ACAT1 ELISA Kit allows for the in vitro quantitative determination of ACAT1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
7.813-500pg/ml
Sensitivity
< 4.688pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of ACAT1 and the recovery rates were calculated by comparing the measured value to the expected amount of ACAT1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-104 |
98 |
|
EDTA plasma(n=5) |
86-103 |
97 |
|
heparin plasma(n=5) |
87-104 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACAT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-101% |
85-98% |
89-105% |
|
EDTA plasma(n=5) |
85-98% |
85-100% |
86-96% |
|
heparin plasma(n=5) |
90-98% |
83-94% |
81-95% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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