FineTest
SKU:EH0777
Human CYP3A4(Cytochrome P450 3A4) ELISA Kit
Human CYP3A4(Cytochrome P450 3A4) ELISA Kit
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CYP3A4|Cytochrome P450 3A4|CYP3A3 CYPIIIA3|CYPIIIA4|Nifedipine|8-cineole 2-exo-monooxygenase|Albendazole monooxygenase|Albendazole sulfoxidase|Cytochrome P450 3A3|Cytochrome P450 HLp|Cytochrome P450 NF-25|Cytochrome P450-PCN1
Product Name
Human CYP3A4(Cytochrome P450 3A4) ELISA Kit
Alias
CYP3A4 ELISA Kit, Cytochrome P450 3A4 ELISA Kit, CYP3A3 CYPIIIA3 ELISA Kit, CYPIIIA4 ELISA Kit, Nifedipine ELISA Kit, 8-cineole 2-exo-monooxygenase ELISA Kit, Albendazole monooxygenase ELISA Kit, Albendazole sulfoxidase ELISA Kit, Cytochrome P450 3A3 ELISA Kit, Cytochrome P450 HLp ELISA Kit, Cytochrome P450 NF-25 ELISA Kit, Cytochrome P450-PCN1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
CYP3A4 ELISA Kit allows for the in vitro quantitative determination of CYP3A4 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of CYP3A4 and the recovery rates were calculated by comparing the measured value to the expected amount of CYP3A4 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
92-102 |
97 |
|
EDTA plasma(n=5) |
94-103 |
99 |
|
heparin plasma(n=5) |
93-104 |
99 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CYP3A4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-103% |
87-105% |
86-93% |
|
EDTA plasma(n=5) |
82-98% |
86-100% |
84-100% |
|
heparin plasma(n=5) |
85-95% |
80-94% |
83-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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