FineTest
SKU:EH0769
Human VGF(Neurosecretory protein VGF) ELISA Kit
Human VGF(Neurosecretory protein VGF) ELISA Kit
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VGF|Neurosecretory protein VGF
Product Name
Human VGF(Neurosecretory protein VGF) ELISA Kit
Alias
VGF ELISA Kit, Neurosecretory protein VGF ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
VGF ELISA Kit allows for the in vitro quantitative determination of VGF concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of VGF and the recovery rates were calculated by comparing the measured value to the expected amount of VGF in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
93-104 |
96 |
|
EDTA plasma(n=5) |
87-102 |
94 |
|
heparin plasma(n=5) |
87-103 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-100% |
91-102% |
85-104% |
|
EDTA plasma(n=5) |
89-101% |
85-101% |
85-100% |
|
heparin plasma(n=5) |
88-100% |
84-90% |
83-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
