FineTest
SKU:EH0634
Human CD276(CD276 antigen) ELISA Kit
Human CD276(CD276 antigen) ELISA Kit
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CD276|B7-H3|B7H3|B7 homolog 3|CD276 antigen|CD276 molecule|Costimulatory molecule
Product Name
Human CD276(CD276 antigen) ELISA Kit
Alias
CD276 ELISA Kit, B7-H3 ELISA Kit, B7H3 ELISA Kit, B7 homolog 3 ELISA Kit, CD276 antigen ELISA Kit, CD276 molecule ELISA Kit, Costimulatory molecule ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
CD276 ELISA Kit allows for the in vitro quantitative determination of CD276 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.781-50ng/ml
Sensitivity
< 0.469ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of CD276 and the recovery rates were calculated by comparing the measured value to the expected amount of CD276 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-105 |
98 |
|
EDTA plasma(n=5) |
87-103 |
95 |
|
heparin plasma(n=5) |
91-104 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD276 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-99% |
93-103% |
87-103% |
|
EDTA plasma(n=5) |
83-100% |
83-100% |
90-99% |
|
heparin plasma(n=5) |
82-96% |
82-95% |
86-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
