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SKU:EH0551

Human HIF1A(Hypoxia-inducible factor 1-alpha) ELISA Kit

Human HIF1A(Hypoxia-inducible factor 1-alpha) ELISA Kit

Regular price $400.00 USD
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size
Sensitivity
Range

HIF-1α|HIF1A|ARNT-interacting protein|Basic-helix-loop-helix-PAS protein MOP1|BHLHE78|Class E basic helix-loop-helix protein 78|HIF-1-alpha|HIF1-alpha|hypoxia-inducible factor 1-alpha|Member of PAS protein 1|member of PAS superfamily 1|PAS domain-containing protein 8

Product Name

Human HIF1A(Hypoxia-inducible factor 1-alpha) ELISA Kit

Alias

HIF-1α ELISA KitHIF1A ELISA KitARNT-interacting protein ELISA KitBasic-helix-loop-helix-PAS protein MOP1 ELISA KitBHLHE78 ELISA KitClass E basic helix-loop-helix protein 78 ELISA KitHIF-1-alpha ELISA KitHIF1-alpha ELISA Kithypoxia-inducible factor 1-alpha ELISA KitMember of PAS protein 1 ELISA Kitmember of PAS superfamily 1 ELISA KitPAS domain-containing protein 8 ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

HIF1A ELISA Kit allows for the in vitro quantitative determination of HIF1A concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Species

Human

UniProt ID

Q16665

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of HIF1A and the recovery rates were calculated by comparing the measured value to the expected amount of HIF1A in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

85-102

90

EDTA plasma(n=5)

89-100

96

heparin plasma(n=5)

93-105

98

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HIF1A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

86-102%

89-104%

94-101%

EDTA plasma(n=5)

82-93%

84-96%

88-100%

heparin plasma(n=5)

86-96%

85-100%

88-99%

CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

 

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