FineTest
SKU:EH0548
Human PDGFB(Platelet-derived growth factor subunit B) ELISA Kit
Human PDGFB(Platelet-derived growth factor subunit B) ELISA Kit
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PDGFB|B chain|Becaplermin|FLJ12858|PDGF subunit B|PDGF2|PDGF-2|platelet-derived growth factor 2|Platelet-derived growth factor beta polypeptide|platelet-derived growth factor subunit B|platelet-derived growth factor|B chain
Product Name
Human PDGFB(Platelet-derived growth factor subunit B) ELISA Kit
Alias
PDGFB ELISA Kit, B chain ELISA Kit, Becaplermin ELISA Kit, FLJ12858 ELISA Kit, PDGF subunit B ELISA Kit, PDGF2 ELISA Kit, PDGF-2 ELISA Kit, platelet-derived growth factor 2 ELISA Kit, Platelet-derived growth factor beta polypeptide ELISA Kit, platelet-derived growth factor subunit B ELISA Kit, platelet-derived growth factor ELISA Kit, B chain ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PDGFB ELISA Kit allows for the in vitro quantitative determination of PDGFB concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PDGFB and the recovery rates were calculated by comparing the measured value to the expected amount of PDGFB in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-103 |
97 |
|
EDTA plasma(n=5) |
87-94 |
90 |
|
heparin plasma(n=5) |
91-103 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDGFB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-104% |
86-105% |
91-105% |
|
EDTA plasma(n=5) |
82-97% |
82-101% |
86-99% |
|
heparin plasma(n=5) |
84-99% |
84-97% |
87-95% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
