FineTest
SKU:EH0532
Human AKT1(RAC-alpha serine/threonine-protein kinase) ELISA Kit
Human AKT1(RAC-alpha serine/threonine-protein kinase) ELISA Kit
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PKB|AKT1|PKB alpha|PRKBA|RAC-alpha|AKT|PKBMGC99656|Protein kinase B|Proto-oncogene c-Akt|rac protein kinase alpha|RAC-ALPHA|RAC-alpha serine|threonine-protein kinase|RAC-PK-alpha|RACPKB-ALPHA|v-akt murine thymoma viral oncogene homolog 1
Product Name
Human AKT1(RAC-alpha serine/threonine-protein kinase) ELISA Kit
Alias
PKB ELISA Kit, AKT1 ELISA Kit, PKB alpha ELISA Kit, PRKBA ELISA Kit, RAC-alpha ELISA Kit, AKT ELISA Kit, PKBMGC99656 ELISA Kit, Protein kinase B ELISA Kit, Proto-oncogene c-Akt ELISA Kit, rac protein kinase alpha ELISA Kit, RAC-ALPHA ELISA Kit, RAC-alpha serine ELISA Kit, threonine-protein kinase ELISA Kit, RAC-PK-alpha ELISA Kit, RACPKB-ALPHA ELISA Kit, v-akt murine thymoma viral oncogene homolog 1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
AKT1 ELISA Kit allows for the in vitro quantitative determination of AKT1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of AKT1 and the recovery rates were calculated by comparing the measured value to the expected amount of AKT1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-101 |
97 |
|
EDTA plasma(n=5) |
87-103 |
95 |
|
heparin plasma(n=5) |
88-101 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AKT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-104% |
93-104% |
87-100% |
|
EDTA plasma(n=5) |
87-97% |
90-101% |
82-96% |
|
heparin plasma(n=5) |
86-100% |
80-91% |
84-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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