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FineTest

EH0468

Human 5-NT(5-Nucleotidase) ELISA Kit

Human 5-NT(5-Nucleotidase) ELISA Kit

$450.00 USD
$450.00 USD
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size
Sensitivity
Range

5-NT|NT5E|5'-NT|CD73|E5NT|eNT|NTE|eN|NT|NT5|5' nucleotidase|CD73|CD73 antigen|Ecto-5'-nucleotidase|5'-nucleotidase|Purine 5-Prime-Nucleotidase

Product Name

Human 5-NT(5-Nucleotidase) ELISA Kit

Alias

5-NT ELISA Kit, NT5E ELISA Kit, 5'-NT ELISA Kit, CD73 ELISA Kit, E5NT ELISA Kit, eNT ELISA Kit, NTE ELISA Kit, eN ELISA Kit, NT ELISA Kit, NT5 ELISA Kit, 5' nucleotidase ELISA Kit, CD73 ELISA Kit, CD73 antigen ELISA Kit, Ecto-5'-nucleotidase ELISA Kit, 5'-nucleotidase ELISA Kit, Purine 5-Prime-Nucleotidase ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

5-NT ELISA Kit allows for the in vitro quantitative determination of 5-NT concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Species

Human

UniProt ID

P21589

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of 5-NT and the recovery rates were calculated by comparing the measured value to the expected amount of 5-NT in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

90-103

97

EDTA plasma(n=5)

91-104

98

heparin plasma(n=5)

86-101

93

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-NT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

88-103%

94-104%

89-96%

EDTA plasma(n=5)

87-96%

82-101%

82-99%

heparin plasma(n=5)

82-100%

80-97%

80-99%

CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

 

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