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SKU:EH0330
Human WISP1/CCN4(WNT1-inducible-signaling pathway protein 1) ELISA Kit
Human WISP1/CCN4(WNT1-inducible-signaling pathway protein 1) ELISA Kit
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WISP1|WISP-1|CCN4|WISP-1i|CCN4WISP1tc|CCN family member 4|Wnt-1-induced secreted protein|WISP1c|WNT1 induced secreted protein 1
Product Name
Human WISP1/CCN4(WNT1-inducible-signaling pathway protein 1) ELISA Kit
Alias
WISP1 ELISA Kit, WISP-1 ELISA Kit, CCN4 ELISA Kit, WISP-1i ELISA Kit, CCN4WISP1tc ELISA Kit, CCN family member 4 ELISA Kit, Wnt-1-induced secreted protein ELISA Kit, WISP1c ELISA Kit, WNT1 induced secreted protein 1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
WISP1/CCN4 ELISA Kit allows for the in vitro quantitative determination of WISP1/CCN4 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of WISP1/CCN4 and the recovery rates were calculated by comparing the measured value to the expected amount of WISP1/CCN4 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-104 |
98 |
|
EDTA plasma(n=5) |
87-104 |
95 |
|
heparin plasma(n=5) |
90-104 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of WISP1/CCN4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-100% |
96-104% |
85-105% |
|
EDTA plasma(n=5) |
84-98% |
83-100% |
86-101% |
|
heparin plasma(n=5) |
83-95% |
81-90% |
80-93% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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