FineTest
SKU:EH0250
Human PUMA(P53 Upregulated Modulator of Apoptosis) ELISA Kit
Human PUMA(P53 Upregulated Modulator of Apoptosis) ELISA Kit
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PUMA|P53 Upregulated Modulator of Apoptosis
Product Name
Human PUMA(P53 Upregulated Modulator of Apoptosis) ELISA Kit
Alias
PUMA ELISA Kit, P53 Upregulated Modulator of Apoptosis ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PUMA ELISA Kit allows for the in vitro quantitative determination of PUMA concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PUMA and the recovery rates were calculated by comparing the measured value to the expected amount of PUMA in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-98 |
94 |
|
EDTA plasma(n=5) |
86-105 |
99 |
|
heparin plasma(n=5) |
91-102 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PUMA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
89-104% |
91-105% |
95-105% |
|
EDTA plasma(n=5) |
85-99% |
83-94% |
84-98% |
|
heparin plasma(n=5) |
80-91% |
81-96% |
80-86% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
