FineTest
SKU:EH0206
Human PIM-1(Serine/Threonine-protein Kinase Pim-1) ELISA Kit
Human PIM-1(Serine/Threonine-protein Kinase Pim-1) ELISA Kit
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PIM-1
Product Name
Human PIM-1(Serine/Threonine-protein Kinase Pim-1) ELISA Kit
Alias
PIM-1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PIM-1 ELISA Kit allows for the in vitro quantitative determination of PIM-1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.180ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PIM-1 and the recovery rates were calculated by comparing the measured value to the expected amount of PIM-1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-105 |
98 |
|
EDTA plasma(n=5) |
85-105 |
94 |
|
heparin plasma(n=5) |
92-104 |
100 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PIM-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-94% |
88-100% |
85-105% |
|
EDTA plasma(n=5) |
82-101% |
85-101% |
82-98% |
|
heparin plasma(n=5) |
81-98% |
83-96% |
80-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
