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SKU:EH0204
Human 15-LO(Arachidonate 15-Lipoxygenase) ELISA Kit
Human 15-LO(Arachidonate 15-Lipoxygenase) ELISA Kit
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15-LO|ALOX15|15-LOX-1|15LOX-1|LOX15
Product Name
Human 15-LO(Arachidonate 15-Lipoxygenase) ELISA Kit
Alias
15-LO ELISA Kit, ALOX15 ELISA Kit, 15-LOX-1 ELISA Kit, 15LOX-1 ELISA Kit, LOX15 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
15-LO ELISA Kit allows for the in vitro quantitative determination of 15-LO concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
62.5-4000pg/ml
Sensitivity
< 37.5pg/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of 15-LO and the recovery rates were calculated by comparing the measured value to the expected amount of 15-LO in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-94 |
90 |
|
EDTA plasma(n=5) |
88-105 |
95 |
|
heparin plasma(n=5) |
94-105 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 15-LO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-102% |
85-103% |
92-98% |
|
EDTA plasma(n=5) |
85-99% |
85-94% |
84-101% |
|
heparin plasma(n=5) |
82-94% |
81-86% |
80-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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