FineTest
SKU:EH0184
Human IL-1α(Interleukin 1 Alpha) ELISA Kit
Human IL-1α(Interleukin 1 Alpha) ELISA Kit
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IL-1α|IL-1alpha|IL1A|IL1-A|IL1|IL1F1|Preinterleukin 1 Alpha|Hematopoietin-1|Pro-Interleukin-1-Alpha
Product Name
Human IL-1α(Interleukin 1 Alpha) ELISA Kit
Alias
IL-1α ELISA Kit, IL-1alpha ELISA Kit, IL1A ELISA Kit, IL1-A ELISA Kit, IL1 ELISA Kit, IL1F1 ELISA Kit, Preinterleukin 1 Alpha ELISA Kit, Hematopoietin-1 ELISA Kit, Pro-Interleukin-1-Alpha ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IL-1α ELISA Kit allows for the in vitro quantitative determination of IL-1alpha concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
3.906-250pg/ml
Sensitivity
< 2.344pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-1alpha and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1alpha in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
91-99 |
95 |
|
EDTA plasma(n=5) |
87-104 |
95 |
|
heparin plasma(n=5) |
86-100 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1alpha and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-101% |
91-100% |
87-97% |
|
EDTA plasma(n=5) |
84-96% |
84-101% |
87-99% |
|
heparin plasma(n=5) |
81-99% |
80-96% |
85-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
