FineTest
SKU:EH0185
Human IL-1β(Interleukin 1 Beta) ELISA Kit
Human IL-1β(Interleukin 1 Beta) ELISA Kit
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IL-1β|IL-1beta|IL1B|IL1-BETA|IL1F2|catabolin
Product Name
Human IL-1β(Interleukin 1 Beta) ELISA Kit
Alias
IL-1β ELISA Kit, IL-1beta ELISA Kit, IL1B ELISA Kit, IL1-BETA ELISA Kit, IL1F2 ELISA Kit, catabolin ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IL-1β ELISA Kit allows for the in vitro quantitative determination of IL-1beta concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
3.906-250pg/ml
Sensitivity
< 2.344pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-1beta and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1beta in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-100 |
94 |
|
EDTA plasma(n=5) |
87-100 |
93 |
|
heparin plasma(n=5) |
87-105 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1beta and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-104% |
89-102% |
86-102% |
|
EDTA plasma(n=5) |
83-101% |
82-97% |
83-96% |
|
heparin plasma(n=5) |
91-98% |
80-100% |
90-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
