FineTest
SKU:EH0179
Human ACTα1(Actin Alpha 1, Skeletal Muscle) ELISA Kit
Human ACTα1(Actin Alpha 1, Skeletal Muscle) ELISA Kit
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ACTα1|ACTA1|ASMA|CFTD|CFTD1|CFTDM|MPFD|NEM1|NEM2|NEM3
Product Name
Human ACTα1(Actin Alpha 1, Skeletal Muscle) ELISA Kit
Alias
ACTα1 ELISA Kit, ACTA1 ELISA Kit, ASMA ELISA Kit, CFTD ELISA Kit, CFTD1 ELISA Kit, CFTDM ELISA Kit, MPFD ELISA Kit, NEM1 ELISA Kit, NEM2 ELISA Kit, NEM3 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
ACTα1 ELISA Kit allows for the in vitro quantitative determination of ACTα1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of ACTα1 and the recovery rates were calculated by comparing the measured value to the expected amount of ACTα1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-102 |
96 |
|
EDTA plasma(n=5) |
88-100 |
94 |
|
heparin plasma(n=5) |
89-104 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACTα1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-103% |
88-104% |
88-102% |
|
EDTA plasma(n=5) |
87-98% |
91-100% |
88-100% |
|
heparin plasma(n=5) |
89-97% |
83-95% |
81-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
