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Human IL-12(Interleukin 12) ELISA Kit
Human IL-12(Interleukin 12) ELISA Kit
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Interleukin 12 p70|CLMF p35|Cytotoxic lymphocyte matuRation factor 35 kDa subunit|IL-12 subunit p35|IL-12|subunit p35|interleukin 12|p35
Product Name
Human IL-12(Interleukin 12) ELISA Kit
Alias
Interleukin 12 p70 ELISA Kit, CLMF p35 ELISA Kit, Cytotoxic lymphocyte matuRation factor 35 kDa subunit ELISA Kit, IL-12 subunit p35 ELISA Kit, IL-12 ELISA Kit, subunit p35 ELISA Kit, interleukin 12 ELISA Kit, p35 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IL-12 ELISA Kit allows for the in vitro quantitative determination of IL-12 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Human
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-12 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-12 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-104 |
91 |
|
EDTA plasma(n=5) |
91-103 |
100 |
|
heparin plasma(n=5) |
88-101 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
98-105% |
92-99% |
88-99% |
|
EDTA plasma(n=5) |
85-96% |
84-101% |
82-95% |
|
heparin plasma(n=5) |
86-94% |
85-96% |
80-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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